5x phusion hf buffer (New England Biolabs)
New England Biolabs is a verified supplier 
New England Biolabs manufactures this product 
97
Structured Review
New England Biolabs
5x phusion hf buffer
5x Phusion Hf Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1704 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5x+hf+buffer/Phusion+HF+Buffer/bio_rxiv__64898__2026__03__16__712010-181-21-33
Average 97 stars, based on 1704 article reviews
5x Phusion Hf Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1704 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5x+hf+buffer/Phusion+HF+Buffer/bio_rxiv__64898__2026__03__16__712010-181-21-33
Average 97 stars, based on 1704 article reviews
5x phusion hf buffer - by Bioz Stars,
2026-09
97/100 stars
Images
Related Articles
Purification:Article Title: Distinct TCR repertoire diversity, distribution, clonality gene segment use and MHC-restriction in foetal and young adult thymus Article Snippet: This was then purified by adding 50 μL of AMPure XP Beads (Beckman Coulter, cat. no: A63881) to the 100 μL ligation product, with some modifications to manufacturer’s instructions: 300 μL of 80% ethanol was used for the washing steps and the product was eluted in 31 μL nuclease-free water. .. The purified ligation product was then amplified in a PCR reaction consisting of 31 μL purified product, 10 μL of Article Title: Molecular epidemiology and genetic diversity of disappearing Plasmodium vivax in southern Thailand. Article Snippet: The PCR products were then purified using the GeneapHlowTM Gel/PCR Kit (Geneaid, Taiwan) according to the manufacturer’s protocol. .. Purified PCR amplicons of both genes containing overhang sequence were pooled and performed the secondround PCR to prepare the DNA library using Phusion High-Fidelity Master Mix with Article Title: Molecular epidemiology and genetic diversity of disappearing Plasmodium vivax in southern Thailand Article Snippet: .. Purified PCR amplicons of both genes containing overhang sequence were pooled and performed the second-round PCR to prepare the DNA library using Phusion High-Fidelity Master Mix with Article Title: Distinct TCR repertoire diversity, distribution, clonality gene segment use and MHC-restriction in foetal and young adult thymus Article Snippet: The purified PCR product was then amplified in a PCR reaction to add indices and Illumina adaptors P5 and P7 and sequencing primer 1 (SP1) sequence. .. The PCR reaction mix consisted of: 31 μL purified product, 2.5 μL of 1 μM SP1 index (mSP1-6N-I- X -αRC1 or mSP1-6N-I- X -βRC1, for TCRα and TCRβ respectively), 2.5 μL of 10 μM SP2 index (P7-L X ), 2.5 μL of 10 μM SP1-P5 primer, 10 μL of Ligation:Article Title: Distinct TCR repertoire diversity, distribution, clonality gene segment use and MHC-restriction in foetal and young adult thymus Article Snippet: This was then purified by adding 50 μL of AMPure XP Beads (Beckman Coulter, cat. no: A63881) to the 100 μL ligation product, with some modifications to manufacturer’s instructions: 300 μL of 80% ethanol was used for the washing steps and the product was eluted in 31 μL nuclease-free water. .. The purified ligation product was then amplified in a PCR reaction consisting of 31 μL purified product, 10 μL of Amplification:Article Title: Distinct TCR repertoire diversity, distribution, clonality gene segment use and MHC-restriction in foetal and young adult thymus Article Snippet: This was then purified by adding 50 μL of AMPure XP Beads (Beckman Coulter, cat. no: A63881) to the 100 μL ligation product, with some modifications to manufacturer’s instructions: 300 μL of 80% ethanol was used for the washing steps and the product was eluted in 31 μL nuclease-free water. .. The purified ligation product was then amplified in a PCR reaction consisting of 31 μL purified product, 10 μL of Polymerase Chain Reaction:Article Title: Distinct TCR repertoire diversity, distribution, clonality gene segment use and MHC-restriction in foetal and young adult thymus Article Snippet: This was then purified by adding 50 μL of AMPure XP Beads (Beckman Coulter, cat. no: A63881) to the 100 μL ligation product, with some modifications to manufacturer’s instructions: 300 μL of 80% ethanol was used for the washing steps and the product was eluted in 31 μL nuclease-free water. .. The purified ligation product was then amplified in a PCR reaction consisting of 31 μL purified product, 10 μL of Article Title: Molecular epidemiology and genetic diversity of disappearing Plasmodium vivax in southern Thailand. Article Snippet: The PCR products were then purified using the GeneapHlowTM Gel/PCR Kit (Geneaid, Taiwan) according to the manufacturer’s protocol. .. Purified PCR amplicons of both genes containing overhang sequence were pooled and performed the secondround PCR to prepare the DNA library using Phusion High-Fidelity Master Mix with Article Title: Molecular epidemiology and genetic diversity of disappearing Plasmodium vivax in southern Thailand Article Snippet: .. Purified PCR amplicons of both genes containing overhang sequence were pooled and performed the second-round PCR to prepare the DNA library using Phusion High-Fidelity Master Mix with Article Title: Simulation-guided tunable DNA probe design for mismatch tolerant hybridization Article Snippet: .. 10 μL PCR reactions were set-up using 2 μL Article Title: Generation of a helper phage for the fluorescent detection of peptide-target interactions by dual-display phages Article Snippet: .. The PCR mixture contained 10 μl of Article Title: Distinct TCR repertoire diversity, distribution, clonality gene segment use and MHC-restriction in foetal and young adult thymus Article Snippet: The purified PCR product was then amplified in a PCR reaction to add indices and Illumina adaptors P5 and P7 and sequencing primer 1 (SP1) sequence. .. The PCR reaction mix consisted of: 31 μL purified product, 2.5 μL of 1 μM SP1 index (mSP1-6N-I- X -αRC1 or mSP1-6N-I- X -βRC1, for TCRα and TCRβ respectively), 2.5 μL of 10 μM SP2 index (P7-L X ), 2.5 μL of 10 μM SP1-P5 primer, 10 μL of Sequencing:Article Title: Molecular epidemiology and genetic diversity of disappearing Plasmodium vivax in southern Thailand. Article Snippet: The PCR products were then purified using the GeneapHlowTM Gel/PCR Kit (Geneaid, Taiwan) according to the manufacturer’s protocol. .. Purified PCR amplicons of both genes containing overhang sequence were pooled and performed the secondround PCR to prepare the DNA library using Phusion High-Fidelity Master Mix with Article Title: Molecular epidemiology and genetic diversity of disappearing Plasmodium vivax in southern Thailand Article Snippet: .. Purified PCR amplicons of both genes containing overhang sequence were pooled and performed the second-round PCR to prepare the DNA library using Phusion High-Fidelity Master Mix with |